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pet28 mhl vector  (Addgene inc)


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    Structured Review

    Addgene inc pet28 mhl vector
    Pet28 Mhl Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+mhl+vector/pET28-MHL+(Plasmid+%2326096)/pm41408833-46-21-25
    Average 94 stars, based on 39 article reviews
    pet28 mhl vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Structural basis for the concerted antiphage activity in the SIR2–HerA system
    Article Snippet: Genes encoding SIR2 and HerA proteins from Paenibacillus sp. 453MF (NCBI Reference Sequences: WP_091160507; WP_091160506) and E. coli (NCBI Reference Sequences: WP_021577683.1; WP_021577682.1) were codon-optimized and synthesized by GENEWIZ. .. The sequence for the full-length SIR2 protein was cloned into the PET28-MHL vector (Addgene #26096) with an N-terminal His tag, and the gene for the full-length HerA protein was cloned into the 2CT-10 vector (Addgene #55209) with an N-terminal His-MBP tag. ..

    Article Title: Structural basis for the concerted antiphage activity in the SIR2-HerA system.
    Article Snippet: Materials and methods Protein expression and purification Genes encoding SIR2 and HerA proteins from Paenibacillus sp. 453MF (NCBI Reference Sequences: WP_091160507; WP_091160506) and E. coli (NCBI Reference Sequences: WP_021577683.1; WP_021577682.1) were codon-optimized and synthesized by GENEWIZ. .. The sequence for the fulllength SIR2 protein was cloned into the PET28-MHL vector (Addgene #26096) with an N-terminal His tag, and the gene for the full-length HerA protein was cloned into the 2CT-10 vector (Addgene #55209) with an N-terminal His-MBP tag. ..

    Clone Assay:

    Article Title: Structural basis for the concerted antiphage activity in the SIR2–HerA system
    Article Snippet: Genes encoding SIR2 and HerA proteins from Paenibacillus sp. 453MF (NCBI Reference Sequences: WP_091160507; WP_091160506) and E. coli (NCBI Reference Sequences: WP_021577683.1; WP_021577682.1) were codon-optimized and synthesized by GENEWIZ. .. The sequence for the full-length SIR2 protein was cloned into the PET28-MHL vector (Addgene #26096) with an N-terminal His tag, and the gene for the full-length HerA protein was cloned into the 2CT-10 vector (Addgene #55209) with an N-terminal His-MBP tag. ..

    Article Title: Structural basis for the concerted antiphage activity in the SIR2-HerA system.
    Article Snippet: Materials and methods Protein expression and purification Genes encoding SIR2 and HerA proteins from Paenibacillus sp. 453MF (NCBI Reference Sequences: WP_091160507; WP_091160506) and E. coli (NCBI Reference Sequences: WP_021577683.1; WP_021577682.1) were codon-optimized and synthesized by GENEWIZ. .. The sequence for the fulllength SIR2 protein was cloned into the PET28-MHL vector (Addgene #26096) with an N-terminal His tag, and the gene for the full-length HerA protein was cloned into the 2CT-10 vector (Addgene #55209) with an N-terminal His-MBP tag. ..

    Article Title: Biochemical and structural studies of NFIA and NFIC reveal a conserved mechanism for specific DNA recognition and provide insight into potential pathogenicity of disease-associated mutations.
    Article Snippet: .. DNA fragments of the human NFIA gene encoding amino acid residues 10–202, 10–205, 10–221, and 10–238, respectively, were cloned into the pET28-MHL vector (Plasmid #26096; Addgene, Watertown, USA), which includes an N-terminal 6×His-tag and a TEV cleavage site. .. DNA fragments of the human NFIC gene encoding amino acid residues 10–175, 10–202, and 10–206, and the human NFIA gene encoding amino acid residues 10–174, respectively, were cloned into the pET28-MKH8-SUMO vector (plasmid#79526; Addgene), which includes an N-terminal 8×His tag, a SUMO fusion, and a TEV protease cleavage site.

    Article Title: Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy.
    Article Snippet: .. TAT-mKV/KV and TAT-mKA/KA were cloned into the pET28-MHL vector (pET28, Addgene plasmid #26096) using standard molecular cloning techniques. ..

    Produced:

    Article Title: Disruption of SETD3-mediated histidine-73 methylation by the BWCFF-associated β-actin G74S mutation.
    Article Snippet: .. Human SETD3 was produced using a plasmid encoding residues 3–502 of human SETD3 (UniProt ID: Q86TU7) with an N-terminal His6-tag in a pET28-MHL vector (plasmid #32873; Addgene, Watertown, MA, USA). ..

    Plasmid Preparation:

    Article Title: Disruption of SETD3-mediated histidine-73 methylation by the BWCFF-associated β-actin G74S mutation.
    Article Snippet: .. Human SETD3 was produced using a plasmid encoding residues 3–502 of human SETD3 (UniProt ID: Q86TU7) with an N-terminal His6-tag in a pET28-MHL vector (plasmid #32873; Addgene, Watertown, MA, USA). ..

    Article Title: Biochemical and structural studies of NFIA and NFIC reveal a conserved mechanism for specific DNA recognition and provide insight into potential pathogenicity of disease-associated mutations.
    Article Snippet: .. DNA fragments of the human NFIA gene encoding amino acid residues 10–202, 10–205, 10–221, and 10–238, respectively, were cloned into the pET28-MHL vector (Plasmid #26096; Addgene, Watertown, USA), which includes an N-terminal 6×His-tag and a TEV cleavage site. .. DNA fragments of the human NFIC gene encoding amino acid residues 10–175, 10–202, and 10–206, and the human NFIA gene encoding amino acid residues 10–174, respectively, were cloned into the pET28-MKH8-SUMO vector (plasmid#79526; Addgene), which includes an N-terminal 8×His tag, a SUMO fusion, and a TEV protease cleavage site.

    Article Title: Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy.
    Article Snippet: .. TAT-mKV/KV and TAT-mKA/KA were cloned into the pET28-MHL vector (pET28, Addgene plasmid #26096) using standard molecular cloning techniques. ..

    Article Title: Structure-Guided DOT1L Probe Optimization by Label-Free Ligand Displacement
    Article Snippet: .. A construct of human DOT1L covering residues 1-420 in the pET28-MHL vector (Addgene 36196) was overexpressed in E. coli BL21 (DE3) in Terrific Broth medium in the presence of 50 mg/ml of kanamycin. ..

    Molecular Cloning:

    Article Title: Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy.
    Article Snippet: .. TAT-mKV/KV and TAT-mKA/KA were cloned into the pET28-MHL vector (pET28, Addgene plasmid #26096) using standard molecular cloning techniques. ..

    Construct:

    Article Title: Structure-Guided DOT1L Probe Optimization by Label-Free Ligand Displacement
    Article Snippet: .. A construct of human DOT1L covering residues 1-420 in the pET28-MHL vector (Addgene 36196) was overexpressed in E. coli BL21 (DE3) in Terrific Broth medium in the presence of 50 mg/ml of kanamycin. ..



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